Homologues of the RPW8 Resistance Protein Are Localized to the Extrahaustorial Membrane that Is Likely Synthesized De Novo.

The Arabidopsis RPW8 resistance protein is recruited to the extrahaustorial membrane of biotrophic powdery mildew fungi.

Specific Targeting of the Arabidopsis Resistance Protein RPW8.2 to the Interfacial Membrane Encasing the Fungal Haustorium Renders Broad-Spectrum Resistance to Powdery Mildew W OA

Powdery mildew fungal pathogens penetrate the plant cell wall and develop a feeding structure called the haustorium to steal photosynthetate from the host cell. Here, we report that the broad-spectrum mildew resistance protein RPW8.2 from Arabidopsis thaliana is induced and specifically targeted to the extrahaustorial membrane (EHM), an enigmatic interfacial membrane believed to be derived from...

Specific targeting of the Arabidopsis resistance protein RPW8.2 to the interfacial membrane encasing the fungal Haustorium renders broad-spectrum resistance to powdery mildew.

Powdery mildew fungal pathogens penetrate the plant cell wall and develop a feeding structure called the haustorium to steal photosynthetate from the host cell. Here, we report that the broad-spectrum mildew resistance protein RPW8.2 from Arabidopsis thaliana is induced and specifically targeted to the extrahaustorial membrane (EHM), an enigmatic interfacial membrane believed to be derived from...

A unique RPW8-encoding class of genes that originated in early land plants and evolved through domain fission, fusion, and duplication

Duplication, lateral gene transfer, domain fusion/fission and de novo domain creation play a key role in formation of initial common ancestral protein. Abundant protein diversities are produced by domain rearrangements, including fusions, fissions, duplications, and terminal domain losses. In this report, we explored the origin of the RPW8 domain and examined the domain rearrangements that have...

Border patrol on the extrahaustorial membrane: Arabidopsis resistance protein RPW8.2 activates targeted, postpenetration defenses.

The spores of powdery mildew fungal pathogens germinate on the leaf surface, attack plants by digesting through the cell wall, and invade the cell by producing a feeding structure, the haustorium (reviewed in Hückelhoven and Panstruga, 2011). The fungus forces changes in the plant cell’s metabolism, cytoskeleton, secretory pathways, and endomembrane system; for example, the fungus reroutes host...